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Biological ferrous sulfate oxidation by A. ferrooxidans immobilized on chitosan beads

Abstract : The immobilization of Acidithiobacillus ferrooxidans cells on chitosan and cross-linked chitosan beads and the biooxidation of ferrous iron to ferric iron in a packed-bed bioreactor were studied. The biofilm formation was carried out by using a glass column reactor loaded with chitosan or cross-linked chitosan beads and 9 K medium previously inoculated with A. ferrooxidans cells. The immobilization cycles on the carrier matrix with the bioreactor operating in batch mode were compared. Then, the reactor was operated using a continuous flow of 9 K medium at different dilution rates. The results indicate that the packed-bed reactor allowed increasing the flow rate of medium approximately two fold (chitosan) and eight fold (chitosan cross-linked) without cells washout, compared to a free cell suspension reactor used as control, and to reach ferric iron productivities as high as 1100 and 1500 mg l− 1 h− 1 respectively. Scanning electron microscopy micrographs of the beads, infrared spectroscopy and the X-ray diffraction patterns of precipitates on the chitosan beads were also investigated.
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A. Giaveno, L. Lavalle, Guibal Eric, E. Donati. Biological ferrous sulfate oxidation by A. ferrooxidans immobilized on chitosan beads. Journal of Microbiological Methods, Elsevier, 2008, 72 (3), pp.227-234. ⟨10.1016/j.mimet.2008.01.002⟩. ⟨hal-03212283⟩

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