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Journal articles
The sf32 Unique Gene of Spodoptera frugiperda Multiple Nucleopolyhedrovirus (SfMNPV) Is a Non-Essential Gene That Could Be Involved in Nucleocapsid Organization in Occlusion-Derived Virions
Abstract : A recombinant virus lacking the sf32 gene (Sf32null), unique to the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), was generated by homologous recombination from a bacmid comprising the complete viral genome (Sfbac). Transcriptional analysis revealed that sf32 is an early gene. Occlusion bodies (OBs) of Sf32null contained 62% more genomic DNA than viruses containing the sf32 gene, Sfbac and Sf32null-repair, although Sf32null DNA was threefold less infective when injected in vivo. Sf32null OBs were 18% larger in diameter and contained 17% more nucleocapsids within ODVs than those of Sfbac. No significant differences were detected in OB pathogenicity (50% lethal concentration), speed-of-kill or budded virus production in vivo. In contrast, the production of OBs/larva was reduced by 39% in insects infected by Sf32null compared to those infected by Sfbac. The SF32 predicted protein sequence showed homology (25% identity, 44% similarity) to two adhesion proteins from Streptococcus pyogenes and a single N-mirystoylation site was predicted. We conclude that SF32 is a non-essential protein that could be involved in nucleocapsid organization during ODV assembly and occlusion, resulting in increased numbers of nucleocapsids within ODVs.
Cited literature [52 references]
https://hal.mines-ales.fr/hal-02949305
Contributor : Administrateur Imt - Mines Alès <>
Submitted on : Friday, September 25, 2020 - 2:49:43 PM
Last modification on : Thursday, April 15, 2021 - 7:18:01 PM
Long-term archiving on: : Thursday, December 3, 2020 - 6:00:03 PM
Contributor : Administrateur Imt - Mines Alès <>
Submitted on : Friday, September 25, 2020 - 2:49:43 PM
Last modification on : Thursday, April 15, 2021 - 7:18:01 PM
Long-term archiving on: : Thursday, December 3, 2020 - 6:00:03 PM
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journal.pone.0077683.PDF
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