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Synthèse peptidique continue, sans solvant et non enzymatique par extrusion réactive

Continuous, solvent-free and non-enzymatic peptide synthesis by reactive extrusion

Abstract : The present disclosure relates to A a continuous, solvent-free and non-enzymatic method for synthesizing a compound of formula (I) :          Ra-POLYPEP-Rc     (I) wherein : POLYPEP is a poly-amino acid compound, Ra and Rc are as specified in the disclosure, which method comprises the steps of: a) feeding an extrusion reactor with (1) a compound of formula (II)          Ra-PEPNt-Rg     (II) wherein; PEPNt is a mono- or a poly-aminoacid compound, Ra and Rg are as specified in the disclosure, and (2) a compound of formula (III)          H-PEPCt-Rc     (III) wherein : PEPCt is a mono- or a poly-amino acid compound, and Rc is as defined in the disclosure in the absence of any solvent, so that the compound of formula (II) and the compound of formula (III) react together for generating a compound of formula (I), and b) collecting the compound of formula (I) from the extrusion reactor.